波士顿儿童医院和哈佛医学院博士后研究员职位–干细胞培养、分子生物学和动物模型方向

DESCRIPTION

The Melero-Martin laboratory seeks highly motivated post-doctoral research fellows to investigate the mechanism by which vascular networks are formed from pluripotent stem cells and to develop novel approaches to therapeutic vascularization and tissue regeneration. We are also looking for candidates to study the use of fate-determining transcription factors to generate human vascular organoids.

Current NIH-funded (R01) projects at the Melero-Martin lab include:

Project 1 – Enhancing endothelial cell engraftment via transplantation of exogenous mitochondria. Goal: To dissect the mechanism by which mitochondria transfer renders cytoprotection in endothelial cells and to elucidate the role of autophagy and mitophagy upon the transfer of mitochondria.

Project 2 – Regulation of endothelial cell specification. Goal: To dissect the activity of the transcription factor ETV2 to drive endothelial cell specification in human pluripotent stem cells.

Project 3 – Vascular networks genetically engineered for protein drug delivery. Goal: To establish the feasibility of using bioengineered drug-secreting vascular networks to treat a clotting deficiency.

Project 4 – Human endothelial cell regulation of ossification. Goal: To elucidate the mechanisms by which blood vessels regulate the osteogenic activity of human mesenchymal stem cells and to develop new therapeutic strategies to regenerate bone.

All work will involve human stem cell culture, molecular biology, and mouse xenograft models.

BACKGROUND READING

Human endothelial colony-forming cells serve as trophic mediators for mesenchymal stem cell engraftment via paracrine signaling. Lin RZ, Moreno-Luna R, Li D, Jaminet SCS, Greene AK, Melero-Martin JM. Proc Natl Acad Sci USA 2014; 111(28):10137-10142.

Host non-inflammatory neutrophils mediate the engraftment of bioengineered vascular networks. Lin RZ, Lee CN, Moreno-Luna R, Neumeyer J, Piekarski B, Zhou P, Moses MA, Sachdev M, Pu WT, Emani S, Melero-Martin JM. Nat Biomed Eng 2017; 1(6):0081.

Robust differentiation of human pluripotent stem cells into endothelial cells via temporal modulation of ETV2 with modified mRNA. Wang K, Lin RZ, Hong X, Ng AH, Lee CN, Neumeyer J, Wang G, Wang X, Ma M, Pu WT, Church GM, Melero-Martin JM. Sci Adv 2020; 6, eaba7606

Bioengineering hemophilia A-specific microvascular grafts for delivery of full-length factor VIII into the bloodstream. Neumeyer J, Lin RZ, Wang K, Hong X, Hua T, Croteau SE, Neufeld EJ, Melero-Martin JM. Blood Adv 2019; 3(24):4166-4176

REQUIREMENTS

• Ph.D., Sc.D., or equivalent degree (or soon to be completed)
• A record of innovative publications (first-authored publications in peer-reviewed journals)
• Experience with stem cell culture, molecular biology, and animal models is highly desirable
• Independent in scientific research and writing, self-motivated, and ethical
• Excellent communication and organizational skills
• Strong interest in regenerative medicine

TO APPLY

Applicants should send a CV, a brief statement of interest, and contact information for three references to Dr. Melero-Martin

(meleromartinlabopportunities@gmail.com). Salary will be commensurate with experience.

Boston Children’s Hospital is an Equal Opportunity / Affirmative Action Employer. Qualified applicants will receive consideration for employment without regard to their race, color, religion, national origin, sex, sexual orientation, gender identity, protected veteran status or disability.

LABORATORY

Melero-Martin Lab

meleromartinlab.com

PI: Juan Melero-Martin, PhD

Boston Children’s Hospital

Harvard Medical School

Harvard Stem Cell Institute

Boston MA, United States